Journal: Scientific Data

Article Title: Benchmarking second and third-generation sequencing platforms for microbial metagenomics

doi: 10.1038/s41597-022-01762-z

Figure Lengend Snippet: Overview of the main sequencing platform characteristics used in this study.

Article Snippet: Technology Type(s) , Illumina HiSeq 3000 • ThermoFisher Ion Proton and GeneStudio S5 • MGI DNBseq G400 and T7 • Oxford Nanopore Sequencing • PacBio Sequel System • Genome mapping and assembly.

Techniques: Sequencing, Amplification

Journal: Scientific Data

Article Title: Benchmarking second and third-generation sequencing platforms for microbial metagenomics

doi: 10.1038/s41597-022-01762-z

Figure Lengend Snippet: Mapping summary per mock and sequencing technology.

Article Snippet: Technology Type(s) , Illumina HiSeq 3000 • ThermoFisher Ion Proton and GeneStudio S5 • MGI DNBseq G400 and T7 • Oxford Nanopore Sequencing • PacBio Sequel System • Genome mapping and assembly.

Techniques: Sequencing

Journal: Scientific Data

Article Title: Benchmarking second and third-generation sequencing platforms for microbial metagenomics

doi: 10.1038/s41597-022-01762-z

Figure Lengend Snippet: Overall comparison between observed and excepted mock compositions per technology. After read mapping to the mock reference genome, a subsampling was performed 3 times at multiple sequencing depth from 10,000 to 1 million reads, except for ONT MinION and PacBio for which maximum depth was 500k. Spearman rank correlations were calculated between observed genomes abundances normalized by genome size (expressed in %) and the expected mock composition (%). Means ± SD are reported based on the 3 iterations performed per depth. PacBio sequencing was not performed on mock3 and mock2, DNBSEQ-T7 and DNBSEQ-G400 were not performed on mock3.

Article Snippet: Technology Type(s) , Illumina HiSeq 3000 • ThermoFisher Ion Proton and GeneStudio S5 • MGI DNBseq G400 and T7 • Oxford Nanopore Sequencing • PacBio Sequel System • Genome mapping and assembly.

Techniques: Comparison, Sequencing, PacBio Sequencing

Journal: Scientific Data

Article Title: Benchmarking second and third-generation sequencing platforms for microbial metagenomics

doi: 10.1038/s41597-022-01762-z

Figure Lengend Snippet: Mock1 metaquast assembly report.

Article Snippet: Technology Type(s) , Illumina HiSeq 3000 • ThermoFisher Ion Proton and GeneStudio S5 • MGI DNBseq G400 and T7 • Oxford Nanopore Sequencing • PacBio Sequel System • Genome mapping and assembly.

Techniques:

Journal: Scientific Data

Article Title: Benchmarking second and third-generation sequencing platforms for microbial metagenomics

doi: 10.1038/s41597-022-01762-z

Figure Lengend Snippet: Shotgun metagenomic datasets description.

Article Snippet: Technology Type(s) , Illumina HiSeq 3000 • ThermoFisher Ion Proton and GeneStudio S5 • MGI DNBseq G400 and T7 • Oxford Nanopore Sequencing • PacBio Sequel System • Genome mapping and assembly.

Techniques: